The road has had to be closed because of the seriousness of the accident and this is expected to bring traffic chaos as thousands of weekend travellers head back to Tokyo on Sunday afternoon.
Our correspondent says there will be serious question about how a major tunnel on one of Japan's most important traffic arteries could have failed so catastrophically, with the private company that runs the highway saying the tunnel had undergone a major inspection just two months ago and had been given a clean bill of health.
Chief Cabinet Secretary Osamu Fujimura told a news conference on Monday: "The prime minister ordered the transport ministry to put the utmost efforts to rescue victims, to quickly investigate the cause of the accident and to establish measures to prevent similar accidents and to provide a counselling service to victims."
On Monday, one of the men will face criminal charges under laws that prohibit against workers from initiating, continuing or participating in illegal strikes. Four men were charged last week.
uthorities said in a statement on Saturday that the work permits of 29 other drivers would be revoked, followed by their deportation. None of the men were identified.
The improving numbers are mostly because of government investment," said Dong Xian'an, economist with Peking First Advisory, about the official numbers.
From the second quarter the government has unleashed a lot of projects, and that has started to be felt in the economy, but it's not a very healthy recovery yet."
One of the reasons is the slowdown in demand for Chinese goods from major markets such as the US, the eurozone and Japan. All are struggling with their own sluggish recoveries from the global economic crisis.
The world is watching. The use of chemical weapons is and would be totally unacceptable," said Mr Obama in a speech at the National Defense University in Washington.